International Issues Essay

Abstract No two countries have the same political and legal system. Each country has its own laws and regulations on business and products. When doing business in another country it is important to know the laws and abide by them. Culture plays a huge part in a business’s success when venturing into a foreign market. Being sensitive to the cultural and religious beliefs in that country is important to a business’s long term success. A business needs to be educated in the laws, customs and cultures of that country so that business will be able to comply and coincide with that country. A business needs to know how and where to resolve any legal issues that may arise while doing business in that country. Disputes need to be handled in a way that is respectful to that country and adheres to the contract in which the parties agreed. International Issues Issues in Legal Disputes in International Transactions â€Å"International transactions and dispute resolution requires both an understanding of the law and a sensitivity to foreign culture, politics, formal and informal power structures and decision-making approaches† (White and Williams LLP, 2013). When entering into a contract with a foreign country, one needs to be very specific in the contract about how to resolve any issues that could come up between the two companies. Deciding and agreeing on where issues will be resolved is a key element in the contract. Going to court can be costly for both companies and the decision made by the court could take an extended amount of time. If the companies were to go to court, deciding in where the court hearing would take place is another issue. If the hearing was to be held in the foreign country, the laws might not be the same and that country might not have much experience in international disputes. If the hearing was to be held in the United States, the other company and the country’s government it resides in could become hostile and not uphold the ruling. Arbitration would be the best way to resolve any disputes between the two countries. Arbitration can save a lot of time and money for both companies while also protecting the images of both companies by not having a lengthy trial in the press. â€Å"Arbitration is often less costly, less litigious, and less time-consuming, and offers more privacy to the parties than litigation† (Companies Export, 2008).The ruling from the arbitration could be binding or non-binding depending on what is agreed upon in the contract. Practical Considerations When trying to resolve a dispute with a company in a foreign country, one would need to look over the written contract that was drawn up at the beginning of the relationship. â€Å"The governing law clause will set out which country’s law will apply to any dispute under the contract, and the jurisdiction clause will state in which country the legal action must be taken† (Smith, 2007). The U.S. based company needs to be aware of the laws that affect the company and the business dealings in the other country. The company needs to consider the cultural aspects, the ethical behavior, and the legal structure within that country. â€Å"U.S. laws governing the international business activities of the U.S. companies fall into two categories. The first consists of laws, such as antitrust, employment, and economic-espionage laws, that are also applied frequently in the domestic context. With respect to these laws, the international business activities of the U.S. companies are a rguably at special risk because management educated and trained outside the United States might be less familiar with these laws than management educated and trained in the United States† (Winer, 2013). Bribery is also a consideration when taking legal action against a foreign business partner because this is illegal under the U.S. Foreign Corrupt Practices Act (FCPA). In some countries, bribery and gift giving is not seen as an illegal or unethical act but a form of showing respect and gratitude. A company needs to be careful on how this is perceived because it could be seen as an illegal act on the company’s part. Factors against CadMex Time and money would work against CadMex in the sublicensing agreements. In the simunlation, Candore is under an epidemic and needs the medicine ViroBlax at a subsidized price. There would not be enough time, due to the epidemic, to work out a contract that both parties will agree upon. Each separate pharmaceutical company in Candore would have to be sought out and a new contract would have to be drawn up for each different company. This is not only costing CadMex time but countless dollars on man hours. CadMex would have to invest a lot of money into properly training the employees and building the proper facilities. CadMex would also have to insure that the generic products were to the proper standards and up to regulation. Even if CadMex was to invest the time and the money into sublicensing the drug, CadMex would lose money because the drug is being sold at a subsidized price. The royalties that would be paid out with sublicensing still would not gap the margin of loss that CadMe x has. When Customs and Laws Conflict When the local customs and the local laws conflict with the customs and laws of an organization operating abroad; the organization should look at the customs and laws in which it is operating under and those should prevail, as long as the company is following the laws in which it is to operate by. When operating abroad, it is important to be aware and sensitive to the customs of that country. Allowing employees to observe religious and cultural holidays will not only keep moral high but also will keep the company within the laws that country has. Knowing the cultural normalities and integrating those into the business model will allow for the employees and members of the community to feel that the business has their interests mind. Being able to comply with the laws of the country in which the business is in while also complying with the laws set by the country, in which the mother company resides, can be a hard line to walk. Complying with the laws of the country in which was agreed upon in the contract at the onset of the partnership would prevail. Making sure to follow the rules of the country that the business is in is important because that is where the business will produce its goods. â€Å"Employees who engage in international business are responsible for knowing and complying with both the laws and regulations of the countries in which the businesses operate and the U.S. law and regulations that apply outside U.S. borders. If U.S. law conflicts with the local customs, or if the local law is more restrictive than U.S. laws or company policies, one must follow the more restrictive law, custom or policy† (Olin, 2012). Resolving Domestic and International Issues Resolving domestic issues can be done through arbitration or through the court system. It can be as simple as suing a party and showing up to court. When trying to resolve an issue domestically, the laws are well known for both parties and the venue in which the issue will be settled is easily accessible to both parties. Resolving an issue internationally is more work involved. The issue has to be resolved through which venue both parties agreed upon when signing the contract. Both companies need to know the laws of that country and travel might be needed. If arbitration was selected, both parties would need to submit all paperwork to a panel of arbitrators and adhere to the judgment in which the panel decides. If one party does not comply with the ruling, then legal action must be taken. Conclusion When doing business internationally, a company needs to be aware of many factors. Conforming to the culture and abiding by the laws that are set in that country are essential to that business and its success. A company needs to know how to resolve issues that will come up in the daily running of the business. Being sensitive to religious and cultural norms will not only help with the moral of employees but the community as well. Adhering to the contract in which the company agreed upon will help avoid any legal action being taken against them. By following the laws and cultures of the country the business resides in will ensure success of the company and future dealings in that country. References Companies Export. (6/4/2008). Retrieved from http://export.gov/regulation/eg_main_018226.asp Olin. (2012). International Business. Retrieved from http://www.b2i.us/profiles/investor/fullpage.asp?BzID=1548&to=cp&Nav=0&LangID

social pariahs Essay

social pariahs Essay social pariahs Essay Olivia Niemann American studies 3rd September 30, 2013 Social pariahs In this day in age social pariahs are all around us in many different ways. A social pariah is someone who is considered an outcast or â€Å"shunned† from a certain group for an undesirable reason or choice he or she has made. With social media and societies standards there will always be a person or group of people that is ostracized for the decisions and choices they have made. Some ways people have been seen as â€Å"social pariahs† or ostracized from the group are for scandalous reasons, race, political views, religious beliefs, and being bold. One example I found was of Tiger Woods who was the number one ranked golf player in the word. In 2009 Tiger woods openly announced to the public about cheating on his wife for 32 months with a cocktail waitress. This was a very devastating social blow to one of the world’s most popular athletes who was considered a marketable family man with two kids and his unblemished social life until the accident. With all the tal k about Woods affair scandal he lost over 25 million dollars in endorsements with companies. His affair caused a very terse life style for Tiger while he was trying to make up for his mistake. Woods scandal was all over the news for many months and was a very hot topic. He was ostracized and shunned from the golf world. Many hyperboles were made before the real facts of the scandal came out, and for a while no one knew what the truth was. He lost many peoples respect for him not only as an athlete but also as a father figure and husband. His loss I endorsements cost him millions. His affair with this woman not only cost him extreme amounts of money and time but the respect and loyalty of his devoted fans and fellow esteemed golf members. Tiger Woods was the center of many lampoons for a while before the scandal died down and blew over. I agree that with fame comes a certain standard you are held to since you are always in the public eye. You’re in a fishbowl world where eve ryone finds out your mistakes and now with social media they spread much faster. I don’t approve of what Tiger Woods did because he broke his promise to his wife and family and with that he had to accept the consequences that came along with his decisions. His consequences were much

What Exactly Is 10X Email With Garrett Moon - CoSchedule Blog

What Exactly Is 10X Email With Garrett Moon Blog How is your social media engagement? Are you getting enough shares, traffic, and leads to justify your social promotion strategy? Or, are you scratching your head, wondering how you’re going to win the uphill battle that is social media reach today? According to a recent study by BuzzSumo, Facebook reach alone has fallen 20% for brands in 2017. Note also, that this is even before Facebook’s recent News Feed overhaul. To me, rather than panicking about abysmal organic (and sometimes paid) social media performance, it’s time to invest even more in email. It’s time for 10x Email.    Im reading What Exactly Is 10X Email? by @garrett_moon via @Why Invest In Email Marketing? Have you heard the stats surrounding email ROI? Email is 40x more effective than social media  for customer acquisition. Email averages a 3800% return on investment. Email inboxes are home to 13 hours of employee’s  time per week. For , our email marketing program has been the biggest driver of marketing success, at an average 300% return. Our experience mirrors the numbers experienced by other companies. And if good data is truth, then it’s time every marketer embraces it as a mainstay of their efforts. So, how can you get the most from email marketing? How To 10x Your Email Marketing Results Email marketing is such an integral part of ’s marketing I devoted an entire chapter to doing it right in my book, 10x Marketing Formula. If you get a chance to pick up the book, awesome. But have no fear, I’ll give you an insider’s look at 10x Email Promotion by sharing an excerpt: To monetize an email list requires that you reverse engineer from paying customer to email subscriber. At , the leading indicator, and most important metric for our marketing team to track for success, is trial signups. We know our trial signups will convert to paid customers at a certain rate. We also know that the more email subscribers we get, the more trial signups we get. And because we meticulously measure all conversions  back to their source, we know exactly how many trial signups every email we send should generate. This is important because it’s debunked a marketing myth. Too many marketers are scared of their email lists. They don’t want to send too many emails for fear their audience will unsubscribe. They believe that audiences are fickle and get scared off easily. However, our experience shows that your audience will tolerate daily emails from you if they’re the right fit. And because you’re a 10x marketer creating competition-free content smack dab in the center of your content core, sending fewer emails is simply poor advice. Here’s how we’ve come to see it. To keep the math simple, let’s say we’ve discovered each email will generate ten trial signups so long as we don’t send more than one email per day. Because we’ve measured extensively, we also know that there’s no major uptick in per-email trial signups by sending fewer emails than one per day. So, for us to send any fewer emails would be a huge waste of this major asset. Now, the reason so many marketers are scared of sending too many emails is because they worry about their unsubscribe rates. But here’s a newsflash: unsubscribe rates don’t matter; revenue matters! Also, our data showed that the increase in unsubscribes due to a greater frequency of daily emails was virtually non-existent. Your email sending frequency should be directly linked to what generates the most revenue. So, when it comes to our email list, we’ve learned to value new subscribers above all else. The more new subscribers we have, the more new customers we have. We believe you’ll find this holds true for you, as well. The real trick is how to build an email list filled with the right audience. And that’s exactly what this stage in the 10x Marketing Formula  is all about. From 0 to 250,000 Subscribers In the past four years, we’ve worked hard to figure out what works and what doesn’t in email list building. Through copious testing, plenty of failure, and eventual hockey-stick growth, we’ve learned exactly what’s worked for us. The following strategies have helped us go from zero subscribers to more than 250,000 since our first blog post in March of 2013. Today, we routinely grow by 4,000-plus subscribers each week. We’ve generated these results because, one, every email is extremely relevant to the problems our audience is trying to solve. And two, each email is directly connected to the value itself provides. This means we can quickly grow a list filled with exactly the right people. It grows fast because the content is so damn good- and it’s monetizable because they’re content consumers who will actually turn into customers. It’s worked for the two reasons prior. And we’ve also found a few tactics that consistently amplify our results to the 10x level: Content upgrades  for every blog post Free tools to help solve major marketing problems that intersect with our product Competition-free content  people are desperate not to miss Now, let’s dig into how we use each tactic, what makes them work, and how you can use them in your marketing context. Content Upgrades A content upgrade is a companion resource to content like blog posts. Often, they are things like templates, calculators, worksheets, or any other document that helps your audience put what you’re teaching them into practice. To use them to build an email list, we gate them behind an email opt-in form. So, they get the resource by paying with an email address. This has become pretty standard practice in content marketing. However, at , we routinely craft such comprehensive content upgrades that we believe people would be willing to pay for them. For example, here’s an example of an eight-piece content upgrade bundle for a single blog post. It’s filled with PDFs, spreadsheet templates, and editable worksheets: [PDF] Email list building tips template to help beginners get started [PDF] Email list building guide to help you implement every tactic you learn throughout this blog post. [Worksheet] Email subject line guide to help you increase your open rates [PDF] 500 words to use in your blog titles (and therefore, your email subject lines) [Spreadsheet] Email subject line A/B test spreadsheet template to help you continually improve your subject lines [PDF] Best time to send email guide to help you reach most of your subscribers according to best practices [PDF] Best day to send email Google Analytics custom report to help you use your own data to know the days of the week when your audience opens your email [Custom Report] Best time to send email Google Analytics custom report to help you send emails at the absolute best times when your audience clicks through to read your content These included guides, spreadsheets, templates, and custom Google Analytics reports are dedicated to helping our readers do absolutely everything we’re about to teach them. A Content Upgrade Bundle As of writing this, we have more than 220 content upgrades available in our blog posts and our Marketing Resource Library. Our content upgrades are a core component of meeting our standard of performance of actionability. They’re always custom designed to contour the content we’re creating. And in total, our content upgrades are responsible for well over one hundred thousand email subscribers. The best part about killer content upgrades is that as long as you’re actually showing people how to solve real problems, the upgrades practically write themselves. All you need to do is format your how-to solutions in a spreadsheet, editable document, or even a printable PDF. If you’re providing actionable value, a content upgrade is a natural result. I know what youre thinking: â€Å"I barely have time to write a blog post, much less create a content upgrade.† But what if you just created one fewer post per week and used that time to create a content upgrade? That trade would be worth it, because your list will grow faster even though you’re publishing one less piece per week. That’s a pretty great deal. You could even follow Pat Flynn’s lead  and create one content upgrade per month that’s incorporated into every post. This is super efficient, super smart, and undeniably effective. The bottom line here is you should weigh content upgrades as heavily important in your content mix. They allow your value to travel farther, make your content more actionable, and help grow email lists. They’re well worth the investment, and too important to skip. Content upgrades are well worth the investment, and too important to skip. #10xEmailTools After content upgrades, our most effective list-building drivers have been free tools. Our top three tools for list building have been Headline Analyzer, Click-To-Tweet  WordPress Plugin, And the Social Message Optimizer. Here are the email subscribers each tool has produced. Headline Analyzer In just twenty-four months, the Headline Analyzer tool has contributed about 20 percent of our total list growth. Subscribers Generated:  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  55,040 Timeframe:  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  24 months WordPress Plugin In 2013, we built a social sharing plugin called â€Å"† for WordPress. It’s a smart little tool that allows anyone with a WordPress blog to craft readymade tweets for their readers to share with just a click or tap. We gave it away for free, and today, it’s used on over ten thousand websites. This has also helped build our email list, because when you give away stuff that’s this good, people want to know what else you have to offer. Subscribers Generated:  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  7,407 Timeframe:  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  4 months Social Message Optimizer Our Social Message Optimizer is a free tool that helps marketers write better messages that boost engagement, build trust, drive traffic, and spark conversions. It allows users to type in their message, select which social network it’s written for, and then let our sophisticated algorithm score it. It helps them capture more eyeballs with their messages, getting even more likes, comments, shares, and clicks. It does all this by optimizing according to proven best practices and real data from 6.9 million social media messages analyzed by our team. Subscribers Generated:  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  1,806 Timeframe:  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  8 months Bonus: Email Subject Line Tester As a bonus, our free Email Subject Line Tester  recently launched! Just like our Headline Analyzer, the Email Subject Line Tester is rocket-fueled by real-world data to analyze, score, and suggest optimization for your email subject lines. This means you can write click-worthy subject lines every single time - for free! With this free tool, you can: Drive  more  opens Get more  clicks Enjoy more  conversions Supercharge your subject lines today with this amazing tool.

Argumentation Essay Example | Topics and Well Written Essays - 750 words - 1

Argumentation - Essay Example Critical analysis of these phenomena allows seeing some common prejudices. Men have the necessity to remain behind the mask of masculinity showing no emotions and being aggressive. Women have second roles and are supposed to be treated accordingly and that is why women have no power. These distorted narrative about man and women create many problems for both sexes as it turns out. And the essay is aimed to show which exact problems appear because of this. Boys are taught from the little age to be real men. However, what exactly being a real man means is usually learnt from media. And children unable to understand what is literal and what is not take everything for granted. For instance, Pixar was shooting cartoons with the leading characters being male only. Lonely male creatures in the company of male characters went off on some quest or fought villains in all the Pixar cartoons. Women played secondary roles or sometimes even become final achievements of the heroes. And boys along with girls learn that women cannot be leaders and cannot dream to be presidents. However, since when leadership skills are about power and not about the ability to think analytically and to find resolutions of the problems? And women are equally good in these tasks. Moreover, boys are taught to avoid emotions as emotions are perceived as female feature. However, avoiding real feelings leads to their suppression and to ruining any boy`s personality. The video â€Å"The mask you live in† shows that the results of such changes in psyche are pitiful. Boys are often diagnosed with behavioral disorders and receive medications as treatment, abuse alcohol and drugs and even commit suicides. They are also are more likely to become criminals since they always need to show their masculinity and aggression. The situation with girls is even more critical. Despite feministic movement girls have to fit the stereotypes of womanliness: be gentle and speechless.

Describe an issue where there is an ethical implication Essay

Describe an issue where there is an ethical implication - Essay Example While academic integrity is a long considered issue within higher education establishments across the globe, its implications in light of the recent economic collapse have taken on a newfound importance. In these regards, the area that has received the most attention has been the connection between professors in United States’ business departments and major Wall-Street banks and brokerage firms that contributed to the economic collapse. Perhaps unique to the university business departments, the professors oftentimes receive substantial sums of money for consulting and preparing reports on these financial institutions. While the notion that business professors are receiving money for consultations by itself is not of major concern, there are substantial ethical implications for this type of collaboration. The first implication is that professors have been indicated to accept money for producing content related to the financial prosperity of a country or region, without indicati ng this in the document itself. The significant ethical implications behind this are that the content is biased yet the professor is using his university’s reputation as a means of supporting his claims.

Entrepreneurship & Small Business Management Duerr's Jam Essay

Entrepreneurship & Small Business Management Duerr's Jam - Essay Example The best time to evaluate environmental conditions is prior to making a location commitment. Many states offer company location incentives. One strategy is to establish enterprise zones in order to bring jobs to economically deprived areas. Sponsored by local city/country governments, these zones lure businesses by offering regulatory and tax relief. In exchange for locating or expanding in these areas, eligible business firms receive total exemption from the property taxes normally assessed on a new plant and equipment for three to five years. Locating in an enterprise zone will not solve problems created by poor management or make up for an ill-conceived idea. However, enterprise zones can be used as catalyst to help, jump-start a small firm. Personal Preference of the Entrepreneur: As a practical matter, many entrepreneurs tend to discount customer accessibility, business environment conditions, and resource availability and consider only their personal preference in locating a business. Often, their personal preference is their home community; the possibility of locating elsewhere never enters their mind. Background: Fred and Mary Duerr founded their jam- and marmalade- making business in Heywood, Lancashire, in 1881. Mary prepared the preserves in her kitchen and using her own family recipes, while Fred delivered the filled jars by handcart to the local Co-operative Society and other shops. In 1884 production was moved into a factory building at Guide Bridge, North Manchester; but the business expanded so rapidly that in 1890 Fred commissioned a new model jam and marmalade factory at Old Trafford. ... expanded so rapidly that in 1890 Fred commissioned a new model jam and marmalade factory at Old Trafford. The site there is still occupied by Duerr's, now under the direction of Fred and Mary's great-grandsons and his two sons. Business Description:F Duerr & Sons was established in 1881. It deals in food conservatives which include products like jams including reduced sugar jams; conserves; fruit spreads; marmalades and reduced sugar marmalades; peanut butter and fruit curds as well as condiments such as apple sauce. The brand is most prominent within the marmalade sector, with almost 6% market share, and 3.2 million in sales. However, its share has declined from almost 9% in the year 2000 to 5.5% in 2004, representing a decline in sales of 42% over the four year period. This is at a much faster rate than the category as a whole of 6.5%. In April 2003, Duerr's introduced Half Sugar marmalade which contains half the sugar but 50% more fruit, which is suitable for diabetics and can be used as part of a calorie controlled diet. In December 2004, Duerr's launched a range of premium marmalades under the name Mary Berry, a well-known cookery writer. The range comprises three flavors: orange & apricot, ruby red grapefruit, and thick cut marmalade with muscovado sugar. The company is attempting to attract new users with new product launches within the marmalade category. Within the jam sector, Duerr's accounts for just 1% of jam sales, less than 1 million in sales. The company is also represented within the peanut butter sector with the Kernel King brand which accounts for just 1% of sales. It is the only other branded lemon curd within

The Intensity Of Agglutination

The Intensity Of Agglutination Antibodies are proteins produced during bodies defence against foreign antigens and they are driven from plasma cells. In the event of an immune response B lymphocytes initiate the production of IgM antibody. In comparison to other immunoglobulins IgM is the largest and earliest antibody available in response to an antigen (Bailey Johnson, 2006). The large structure of this antibody is because it consists of an additional domain in its constant area (Overfield et al, 2007). This antibody has a polymeric structure it consists of heavy and light chains. The binding between two heavy chains or between heavy and light chains is facilitated via the disulphide bond. IgM antibody has a pentameric structure consisting of five subunits. These subunits are joined together via a disulphide bond which occurs between the Fc region and the intersubunit, interasubunit- J chain. Two fab antigen binding sites are available on each IgM monomer and since IgM has a pentameric structure ten Fab antigen binding sites are available that can potentially interact with ten antigens (Overfield et al, 2007)(Khurana, 2006). The initial aim of this practical was to discover if red blood cell antigens can interact with IgM anti-D (Rh) antibody and weather as a consequence of this interaction agglutination occurs. The second aim was to discover weather dithiothreitol (DTT) reducing agents is capable of altering the structure of IgM antibody at different concentration hence affecting the level of agglutination and finally to discover if indirect anti-IgM antibody is capable of facilitating agglutination. The large and pentameric structure of IgM antibody can potentiate the possibility of its interaction with red blood cell antigens resulting in formation of agglutination. Material Method For instructions on how to conduct the experiment with the relevant materials used please refer to the practical schedule. The concentrations of DTT added to the nine tubes where as following (0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009 0.01). Results Table 1: The above table illustrates the number of tubes labelled from 1-10 and the concentrations of DTT in (Mol/L). As illustrated in the above table the control tube which is tube 1 lacked DTT while tubes which were numbered as (2, 3, 4, 5, 6 7) consisted of different concentrations of DTT as shown here (0.001, 0.002, 0.003, 0.004, 0.005, 0.006 0.007). According to the first observation results tubes numbered 1-7 expressed signs of agglutination as indicated by a positive sign (+). Instead tubes numbered (8, 9 10) which had the following DTT concentrations (0.007, 0.008 0.009) expressed no indications of agglutination hence they were marked as negative (-). Due to time limitations results for the second antibody labelling could not be obtained. Discussion The intensity of agglutination in these tubes depended on the concentration of DTT. The control tube which is tube 1 is DTT deficient which is accompanied with agglutination. Tubes labelled 2-7 express different concentrations of DTT starting from the lowest hence escalating slowly. In these tubes agglutination is still observed since the effect of DTT is still not strong enough to break the bonds expressed in IgM antibody while as the concentration of DTT escalates further in tubes 8-10 agglutination is not evidenced. DTT is a reducing agent capable of mediating intersubunit and interasubunit-J chain cleavage hence facilitating IgM subunit (Â µ2ÃŽÂ »2) synthesis (Kownatzki Drescher, 1973). As the concentration of DTT escalates its capability to break these bonds with greater intensity increases as seen in tubes 8-10 leading to greater IgM subunit formation (Â µ2ÃŽÂ »2) and lessens the possibility of antigen antibody interaction hence lack of agglutination. In addition DTT a ffects the structure of IgM heavy and light chains by preventing them from unfolding and causes this chain too separate accordingly leading to agglutination deficiency. A continuous raise in DTT concentration as evidenced in tubes 8-10 causes a decline the probability of disulphide bonds from resuming their function in IgM antibody (Valetti Sitia, 1994). According to the study conducted by (Marrodan et al, 2001 Morris et al, 1974) DTT reducing agent restrains agglutination from occurring by facilitating the disulphide bond located in the IgM antibody to break. In addition the 19 S IgM antibody is cleaved by DTT into a 7S subunit. The 7S antibody subunits are rendered incapable of maintaining IgM antibodys function and therefore wont be able to interact with red blood cell antigens leading to lack of agglutination (Knight, 1978). Due to time limitation for the experiment results for the second antibody labelling could not be obtained. According to (Overfield et al, 2007) the lacking agglutination as a consequence of DTT effect can be reversed by adding anti-IgM antibody hence signs of agglutination will appear but the extent of agglutination will depend on whether the IgM antibody subunits have maintained their ability to bind to red blood cells antigen or due to high level of DTT concentration they have been completely deformed. According to the study conducted by (Emmerich et al, 2006) IgM antibody can be used in the diagnosis of Lassa virus infection which is highly predominant in Western African patients. This diagnosis is achieved via using reverse enzyme immunoassay (ELISA) technique to identify anti-Lassa IgM antibody. The result of this study implemented that via using reverse ELISA in 20 patients with sign of fever high level of anti-Lassa IgM antibody was diagnosed indicating the presence of the Lassa virus. In a study conducted by (Varsano et al, 1995) the presence of IgM antibody against respiratory syncytial virus antigen (RSV) was examined in 145 patients via using the ELISA technique. According to the result of this study ELISA-IgM antibody detection is a highly efficient method in the diagnosis of RSV at early stage of the disease. In another study by (Tsuda et al, 2001) polymerase chain reaction (PCR) technique was used to detect for the presence of IgM antibody against TT virus (TTV) in the diagnosis of human circovirus. The result of this experiment suggests that healthy volunteers were defective of anti-TTV IgM antibody whereas infected individuals showed signs of its presence suggesting that this method is beneficial for diagnosis purposes of human circovirus. Immunoglobulin cleavage can be triggered via the action of different enzymes or chemicals. Papain is an enzyme that cleaves IgG antibody into three segments of FC, heavy and light chains. Furthermore IgM antibody can be cleaved by pepsin enzyme either into an antibody that weights less accompanied with FC fragments (Rudmann, 2005)(Svehag et al, 1969). Protease enzyme is driven from Neisseria gonorrhoeae bacteria capable of cleaving IgA antibody (Pouedras et al, 1992). According to (Akesson et al, 2006) streptococcus pyogenes bacteria is responsible in mediating diseases such as gotonsillitis, septicaemia and it intervenes its action by causing IgG antibody cleavage via using an enzyme called Ides. The action of this virus is to insure that the antibody is unavailable to destroy the bacteria. Furthermore trypsin is another enzyme capable of cleaving IgM antibody at temperature above 50 C leading to different FC fragment synthesis (Andrew et al, 1970). Conclusion Normally red blood cell antigens are capable of interacting with IgM antibody resulting in agglutination while in the presence of DTT reducing agent this binding is inhibited leading to lack of agglutination. The extent of this inhibition will depend on the concentration of DTT and the extend of IgM J chain, interchain intrachain cleavage via DTT. The greater the concentration of DTT the stronger its effect is on this chain which lessens the likelihood of this chain regaining their binding capacity hence their ability to regain antigen binding activity. The concept of antigen antibody binding can be used for the diagnosis purpose of many diseases.